BS Abdur Rahman University Summer training cum Internship Program on Phytocompounds Isolation & Analysis by HPLC | 1-19 June 2015

About the School

School of Life Sciences has been established with twin objectives of making the campus cross disciplinary and initiating teaching, research and training programs across the areas of life sciences. The school has been visualized to emerge as a hub for state of the art research in all fields related to Molecular Biology, Bio-Chemistry, Microbiology, Bio-Technology, Cancer Biology, and Genetics etc., through sponsored projects from industries and Government agencies. It has also been visualized to be a training centre for scientists, researchers, clinical staff of hospitals, industry workers in advanced laboratory techniques with hands on experience, through short term intensive programs. The school offers Undergraduate, Postgraduate and Doctoral programmes in Life Sciences. For more information please visit www.bsauniv.ac.in

About the workshop

The  workshop on Phytocompounds Isolation & Analysis by HPLC is  exclusively  designed  for  college  students  pursuing courses  in  life sciences  in various levels including Under  graduation, Post-graduation and Research. This workshop will provide a unique opportunity for students to get introduced to the field of  phytochemistry. Phytcompounds are found in plants which are utilised as drugs in natural medicine. The workshop  includes  lectures  on  basics  as well  as technical aspects of phytocompounds analysis  followed  by  hands  on  training to  practice  the  earned  knowledge. This workshop will also provide the opportunity to visit and use the state of the art research facilities in the School of Life Sciences  at B. S.  Abdur Rahman University. An additional opportunity will be provided to the participants to analyse their own samples used in their research/project*.

* Conditions apply

Programme schedule

Day 1: Extraction of plant material- Collection, identification and authentication of plant materials, specimen preparation, hot maceration and cold maceration, and concentration of plant extracts.

Day 2: Biochemical analysis of extract I- Qualitative analysis of primary and secondary metabolites in extract.

Day 3: Biochemical analysis of extract II- Quantitative analysis of primary metabolites (proteins, lipids and carbohydrates) in extract.

Day 4: Biochemical analysis of extract III- Quantitative analysis of secondary metabolites in extract. Secondary metabolites includes phenols, tannins, flavonoids, etc.,

Day 5: Chromatography - Theory course on chromatography, types, applications and troubleshooting etc.,

Day 6: Column Chromatography I- Solvent-solvent partition chromatography, concentration of fractions and silica gel packing for column chromatography.

Day 7: Column Chromatography II- Compound isolation by Colum chromatography, collection of elution with various solvents and concentration of eluted compounds.

Day 8: Column Chromatography III- Compound purification by Colum chromatography, collection of elution with various solvents and concentration of eluted compounds.

Day 9: Thin layer chromatography analysis of eluted compounds using silica plates.

Day 10: Spectrophotometric analysis of isolated compounds.

Day 11: HPLC I- Theory course on High performance liquid chromatography (HPLC), operations of instrument and software.

Day 12: HPLC II- High performance liquid chromatographic analysis and quantification of phyto-compounds.

Day 13: Fourier transform infrared spectroscopy (FTIR analysis of isolated compound, result analysis and validation.

Day 14: Report preparation.

Day 15: Acquired knowledge assessment by written test and rapport of workshop

Course fee: 10,000/- per participant. Fee includes workshop kit, manual, chemicals and other consumables and refreshment. Group registrants will get 10% discount.

Eligibility: Under graduate students, post graduate students, research scholars and anyone interested in learning.

Instruction to participants: Total number of participants will be 20; 4 students per team; each team can bring their own samples for analysis. Students should bring white lab coat, all the other requirements will be provided by the university during the workshop. Boarding and lodging can be arranged based on request. Three best performing students during the workshop and assessment will be given appreciation certificate in addition to participation certificate.

Fee Payment details:

For Account Transfer: Indian Overseas Bank, Acc No. 165701000019853; IFSC code: IOBA0001657 in favour of Dean, School of Life Sciences.

For demand draft: DD should be drawn in favour of Dean, School of Life Sciences, B.S. Abdur Rahman University payable at Vandalur, Chennai.

For cheque: Cheque towards Dean, School of Life Sciences, B.S. Abdur Rahman University payable at Vandalur, Chennai.

Venue

School of Life Sciences (SLS) B.S.Abdur Rahman University, Vandalur, Chennai - 48.

Landmark: Adjacent to Anna zoological park.

For further communications contact

Organizing Secretary

Prof. S. Hemalatha                                       

Dean School of Life Sciences (SLS)

B.S.Abdur Rahman University, Chennai

Phone: 044‐22759223 Ext. 195 Email: dean.sls@bsauniv.ac.in

courses.sls@bsauniv.ac.in

Co-organiser

Dr. M. K. Sangeetha

Asst. Prof., School of Life Sciences (SLS)

B.S.Abdur Rahman University, Chennai

Phone: 044‐22759223 Ext. 466

Email: sangeetha@bsauniv.ac.in

Important Dates:

Registration: 25/05/2015

Participation confirmation: 28/05/20115

Workshop date: 01/06/2015 to 19/06/2015

Registration form

Name…………………………………………………………………………..........................…………………….

Gender…….…………………………………………………………………………………………………………………

Specialization ………………………………………………………………………………………………………………

Semester ………..………………………………………………………………………………………………....………

Affiliation………………………………………………………………………………………………………......……… Tel……………………..…………… Mobile no………………………………………………………………......……

E-Mail………..………………………………………………………………………………………………………………

Details of fee payment: DD Cheque Account transfer

Payment reference number and date:

Short write up on, why you want to attend the workshop and how this workshop will benefit you (maximum 50 words)

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Participant’s signature                                                HOD/Principal  Signature   with seal