Life Science Incubator Hyderabad Hands on Training in Recombinant DNA Technology/high end Analytical Instruments

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Life Science Incubator, IKP Knowledge Park Training Programme

Life Science Incubator, IKP Knowledge Park aims at imparting training through Analytical Instrumentation Facility onsite. Using latest technologies in the area of Biotechnology, hands on training in molecular biology has been introduced. By providing hands-on experience on various techniques to the students, Life Science Incubator hopes to improve the knowledge and skill set of students thereby developing a pool of trained employees with transferable skills to the industry.

This two weeks programme provides advanced training using latest technologies with hands on experience and demonstrations. This program intends to bridge the gap between academic training and industry needs in the area of Biotechnology and Pharmaceutical Sciences, which are in need of skilled people with hands-on experience. Topics to be covered:

Scheme I- Hands on training in recombinant DNA technology and protein expression

Laboratory Experiments (hands on): Choose either (a) or (b) option :

(a) Hands on training in recombinant DNA technology 

Lab Fundamentals and biochemical calculations 

Preparation of media, reagents, plates 

Sterilization solutions / materials 

Amplification of gene by PCR 

Agarose gel electrophoresis 

Preparation of vector and insert DNA by restriction digestion 

Purification of DNA fragment from agarose gel 

UV Spectrophotometry (DNA quantification) 

Ligation of vector and insert DNA 

Preparation of Competent cells by Calcium chloride method 

Transformation into bacteria (E. coli), screening of recombinant clones by PCR 

SDS-PAGE Experimental design and result analysis.

(b) Hands on training in recombinant protein expression 

Lab Fundamentals and biochemical Calculations 

Preparation of media, reagents 

Sterilization solutions / materials 

Preparation of Competent cells by Calcium chloride method 

Transformation into bacteria (E. Coli, BL21), 

Over expression of recombinant protein (IPTG induction) 

Checking protein expression by SDS-PAGE (MW determination and purity by SDS – PAGE) 

UV Spectrophotometry (Protein quantification) 

Western Blot 

Purity checking by HPLC 

Molecular weight determination by Mass spectrometry

Course Duration: 2 Weeks

Course Fee for Scheme I: Rs.10,000 + service tax Course

Scheme II- Advanced training on high end Analytical Instruments

Choose either (a) or (b) option :

a) NMR

b) DSC, TGA, Particle Size Analyser, Mass Spectrophotometer, FTIR Tentative

Duration: 2 Weeks

Course Fee for Scheme II: Rs.10,000 + service tax

Date: Starts from May 2015 Time: 09:30 A.M. to 06:00 P.M. Monday to Friday

Venue: Life Science Incubator- IKP Knowledge Park, Hyderabad.

Eligibility: B.Tech, B.Sc, B.Pharm, M.Sc, M.Pharm, M.Tech Course Structure: 30% Lecture and 70% Lab

Course fee: Rs.10, 000 (i.e. Rs.10, 000/- fee + Service Tax) per person for each scheme of the course.

Features & Benefits: - Certificate of Course Completion. - Mini Project work.

For Registration: Please contact,

Mr. Amit Tambvekar at training@ikpknowledgepark.com

Dr. Sangita Sen Majee at sangita@ikpknowledgepark.com

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