NATIONAL Workshop on NEXT GENERATION SEQUENCING
April 18-24, 2016
Institution of Excellence (IOE), University of Mysore, Mysuru-570 006
In Association With
Life technologies, Thermo Fisher Scientifics and GenEclat Technologies
AIM AND SCOPE OF THE WORKSHOP:
The “Next Generation Sequencing” (NGS) Workshop is organized to provide postgraduate-level students and researchers in Biological Sciences, with basic knowledge of NGS technologies and platforms and develop an understanding of important aspects of NGS studies, with basic knowledge of the applications and limitations of NGS technologies. The workshop includes lectures on practical and theoretical aspects of Next-generation sequencing (NGS) given by internationally recognized experts followed by hands-on training sessions. The format and content of the Workshop is tailored to the needs of the application scientist routinely dealing with challenges of instrumental procedures, sample preparation, data collection and quantification, as well as those of research workers keen to obtain a deeper understanding of the Chemistry underlying the Next-generation sequencing (NGS), imaging and analysis.
Four days of workshop would focus on understanding the basics of Next-generation sequencing (NGS).
The emphasis would be on operation of Next-generation sequencing (NGS) instruments and sample preparation, run the sample, data collection and analysis for Next-generation sequencing (NGS).
Ion torrent next generation sequencing instruments available at IOE are from life technologies. Two popular Ion Torrent products are Ion Proton system and Ion PGM system. Ion Torrent’s first instrument, the Ion Personal Genome Machine (PGM), is the least expensive next generation sequencer. Given its output capability (currently up to 1 GB) and speed of runs (2 hours), it is being targeted towards smaller genomes and targeted sequencing. It uses disposable chips which come in three varieties of increasing
output. Ion Proton, which allows for larger chips with higher densities needed for exome and whole genome sequencing. The Ion Proton is substantially more expensive, but is capable of generating much larger outputs. The first chip, the PI, is able to generate ~10 GB per run.
NGS Workshop aims to provide useful step-by-step guided hands-on training for entry-level researchers who intend to broaden or venture their research in NGS genomics studies. The workshop is specially structured into easily followed and organized modular format to build basic understanding, deliver key concepts and walk through complex genome informatics analysis in an interactive mode.
1. Introduction to Next-generation sequencing (NGS)
2. Principle, Instrumentation and Application of Next-generation sequencing (NGS)
3. Sample preparation
4. Run the Sample
5. Data collection
6. Data Analysis
7. Interpretation of data