TRPVB Hands-on Flow Cytometry Workshop - 2017 | 24 - 26 July 2017 ~ helpBIOTECH

28 June 2017

TRPVB Hands-on Flow Cytometry Workshop - 2017 | 24 - 26 July 2017


Hands-on Flow Cytometry Workshop - 2017

TRPVB conducts a series of three-day workshops on “Veterinary Clinical applications of Flow cytometry / Confocal microscopy / In vivo imaging / 2D gel electrophoresis”, one each every year. The first workshop for the year 2017 would be on “Veterinary Clinical applications of Flow cytometry” involving basic techniques of flow cytometry and its clinical applications. This workshop at TRPVB will provide a good learning platform for different facets of flow cytometry applications under the accomplished faculty members of TRPVB and external experts.

This workshop would be held from 24/07/2017 to 26/07/2017

TRPVB invites applications for the 3-day hands on training on basic applications of flow cytometry. The training course gives an opportunity for the participants to have hands-on training on immune phenotyping, autoimmune disease analysis and semen characterization and analysis.

Schedule

DAY 1
TIME
THEORY TOPIC
PRESENTER
10.00 A.M to 10.10 A.M
Welcoming Participants
Dr. K. Kumanan
10.20A.M to 11.20 A.M
Basics of flow cytometry
Dr. G. Dhinakar Raj
11.30 A.M to 12.30 P.M
Instrumentation of Moflo XDP high speed cell sorter with compensation technique
Dr.V.Maroudam / Dr. K.Chitra / Mr. Seenuvasan 
PRACTICAL SESSION
12.30 A.M to 01.30 P.M
Hands on Moflo XDP high speed cell sorter
 Dr. K.Chitra  /Mr. Seenuvasan 
2.00 P.M to5.00 P.M
Beads analysis and calibration of instrument
Dr. M. Amitav  /Dr. K.Chitra /
Mr. Seenuvasan 
DAY 2
TIME
THEORY
PRESENTER
10.00 A.M to 11.30 A.M
Multiplexing and Compensation
Dr. M. Amitav 
PRACTICAL SESSION
PRESENTER
11.30A.M to 01.00 P.M
Autoimmune disease analysis
Dr.K.Chitra/P.Valentina Claudet
02.00 P.M to 03.00 P.M
Immunophenotyping
Dr. M. Amitav/ Dr.V.Maroudam
03.00P.M to 04.00 P.M
Immunophenotyping
Dr.K.Chitra /Dr. M. Amitav
04.00 P.M to 05.00 P.M
Results and interpretation using FACS -Moflo-XDP.
Dr.V.Maroudam /Dr. M. Amitav 
DAY 3
TIME
THEORY
PRESENTER
10.00 A.M to 11.30 A.M
Flow cytometry applications in semen biology
Dr.V.Maroudam
PRACTICAL SESSION
PRESENTER
11.30 A.M to 01.00 P.M
Sperm viability - Propidium Iodide (PI), (SYBR 14 / PI), Acrosomal integrity (FITC-PNA/ PI),    Sperm Chromatin structure assay (Acridine Orange)
Dr.K.Chitra/Dr.V.Maroudam
02.00 P.M to 03.00 P.M
Protein Biomarkers for Sperm Quality 
Dr.V.Maroudam / Dr.K.Chitra
03.00 P.M to 04.00 P.M
Membrane potential  ( Mito-tracker deep red), Oxidative stress analysis using Cell –Rox dye
Dr. M. Amitav / P.Valentina Claudet
04.00 P.M to 05.00 P.M
Results and interpretation using FACS - Moflo-XDP.
Dr.V.Maroudam /Dr.M.Amitav
Venue

Translational Research Platform for Veterinary Biological (TRPVB)

Central University Laboratory, CAHS, TANUVAS

Madhavaram Milk Colony, Chennai- 600 051, India.


Phone: 91-44-25556275, 25556276 E-mail: dirtrpvb@tanuvas.org.in


Registration Details

·         No of participants: 10 (maximum)

·         Registration fees: Rs.5000/- (ONLY BY DD / NEFT)

·         Registration will be on First-Come-First-Serve basis

·         No TA/DA or accommodation and transport facility will be provided.
·         Taxes if any would be charged extra.

For NEFT transfer:

Bank Account Name: Director, TRPVB
Branch: Madhavaram, Chennai – 51
Bank Account No.332902010721712
IFSC Code: UBIN0533297

Application process: The interested participants should submit the scanned application form to mail dirtrpvb@tanuvas.org.in  before 15/07/2017. The participants need to take care of their travel and accommodation on their own. The candidates would be informed of their selection by E-mail. Upon receipt of their course fees within the stipulated time in the form of DD in favour of The Director, TRPVB payable at Chennai for Rs. 5000/- their workshop slot would be confirmed.

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