TRPVB Hands-on Flow Cytometry Workshop - 2017 | 24 - 26 July 2017

Hands-on Flow Cytometry Workshop - 2017

TRPVB conducts a series of three-day workshops on “Veterinary Clinical applications of Flow cytometry / Confocal microscopy / In vivo imaging / 2D gel electrophoresis”, one each every year. The first workshop for the year 2017 would be on “Veterinary Clinical applications of Flow cytometry” involving basic techniques of flow cytometry and its clinical applications. This workshop at TRPVB will provide a good learning platform for different facets of flow cytometry applications under the accomplished faculty members of TRPVB and external experts.

This workshop would be held from 24/07/2017 to 26/07/2017

TRPVB invites applications for the 3-day hands on training on basic applications of flow cytometry. The training course gives an opportunity for the participants to have hands-on training on immune phenotyping, autoimmune disease analysis and semen characterization and analysis.

Schedule

DAY 1
TIME	THEORY TOPIC		PRESENTER
10.00 A.M to 10.10 A.M	Welcoming Participants		Dr. K. Kumanan
10.20A.M to 11.20 A.M	Basics of flow cytometry		Dr. G. Dhinakar Raj
11.30 A.M to 12.30 P.M	Instrumentation of Moflo XDP high speed cell sorter with compensation technique		Dr.V.Maroudam / Dr. K.Chitra / Mr. Seenuvasan
PRACTICAL SESSION
12.30 A.M to 01.30 P.M	Hands on Moflo XDP high speed cell sorter		Dr. K.Chitra  /Mr. Seenuvasan
2.00 P.M to5.00 P.M	Beads analysis and calibration of instrument		Dr. M. Amitav  /Dr. K.Chitra / Mr. Seenuvasan
DAY 2
TIME	THEORY		PRESENTER
10.00 A.M to 11.30 A.M	Multiplexing and Compensation		Dr. M. Amitav
PRACTICAL SESSION			PRESENTER
11.30A.M to 01.00 P.M	Autoimmune disease analysis		Dr.K.Chitra/P.Valentina Claudet
02.00 P.M to 03.00 P.M	Immunophenotyping		Dr. M. Amitav/ Dr.V.Maroudam
03.00P.M to 04.00 P.M	Immunophenotyping		Dr.K.Chitra /Dr. M. Amitav
04.00 P.M to 05.00 P.M	Results and interpretation using FACS -Moflo-XDP.		Dr.V.Maroudam /Dr. M. Amitav
DAY 3
TIME		THEORY	PRESENTER
10.00 A.M to 11.30 A.M		Flow cytometry applications in semen biology	Dr.V.Maroudam
PRACTICAL SESSION			PRESENTER
11.30 A.M to 01.00 P.M		Sperm viability - Propidium Iodide (PI), (SYBR 14 / PI), Acrosomal integrity (FITC-PNA/ PI),    Sperm Chromatin structure assay (Acridine Orange)	Dr.K.Chitra/Dr.V.Maroudam
02.00 P.M to 03.00 P.M		Protein Biomarkers for Sperm Quality	Dr.V.Maroudam / Dr.K.Chitra
03.00 P.M to 04.00 P.M		Membrane potential  ( Mito-tracker deep red), Oxidative stress analysis using Cell –Rox dye	Dr. M. Amitav / P.Valentina Claudet
04.00 P.M to 05.00 P.M		Results and interpretation using FACS - Moflo-XDP.	Dr.V.Maroudam /Dr.M.Amitav

Venue

Translational Research Platform for Veterinary Biological (TRPVB)

Central University Laboratory, CAHS, TANUVAS

Madhavaram Milk Colony, Chennai- 600 051, India.

Phone: 91-44-25556275, 25556276 E-mail: dirtrpvb@tanuvas.org.in

Registration Details

·         No of participants: 10 (maximum)

·         Registration fees: Rs.5000/- (ONLY BY DD / NEFT)

·         Registration will be on First-Come-First-Serve basis

·         No TA/DA or accommodation and transport facility will be provided.

·         Taxes if any would be charged extra.

For NEFT transfer:

Bank Account Name: Director, TRPVB

Branch: Madhavaram, Chennai – 51

Bank Account No.332902010721712

IFSC Code: UBIN0533297

Application process: The interested participants should submit the scanned application form to mail dirtrpvb@tanuvas.org.in  before 15/07/2017. The participants need to take care of their travel and accommodation on their own. The candidates would be informed of their selection by E-mail. Upon receipt of their course fees within the stipulated time in the form of DD in favour of The Director, TRPVB payable at Chennai for Rs. 5000/- their workshop slot would be confirmed.