Workshop on Functional Proteomics and Multiple Post-Translational Modification analysis | October 25-28, 2017


Workshop on Functional Proteomics and Multiple Post-Translational Modification analysis

October 25-28, 2017
Center for Systems Biology and Molecular Medicine (CSBMM) at Yenepoya Research Centre is an advanced multidisciplinary research center with state-of-the-art infrastructure to carry out genomics, proteomics and metabolomics investigations. As a part of the academic program of Yenepoya University, scientists at CSBMM will share their expertise in NextGen sequencing-based genomics, high-resolution mass spectrometry-based proteomic and metabolomic technologies with young researchers in India, through a series of workshops. The organizing team of this workshop have successfully conducted over 20 national workshops on high-resolution mass spectrometry-based proteomics. Several faculty and students from reputed Research Institutes and Universities from India have participated in these workshops.

Venue: Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya University

Schedule

Workshop Program
Day 1:
Tutorial Lectures

  • Introduction to proteomics
  • Sample preparation strategies
  • Fractionation strategies (SCX/bRPLC)
  • Day 2:
    Tutorial Lectures

  • Basics of LC-MS/MS analysis
  • LC-MS/MS operation
  • Key concepts of mass spectrometry data analysis
  • Sample preparation II (peptide extraction, C18 clean-up)
  • Day 3:
    Tutorial Lectures

  • Quantitative proteomics
  • Proteomic strategies for PTM analysis
  • TMT labelling
  • Day 4:
    Tutorial Lectures

  • Biological interpretation of proteomics data
  • Validation of proteomic data
  • Hands on training

  • Sample preparation I (In-gel and in-solution digestion)
  • Hands on training

  • Sample preparation II (peptide extraction, C18 clean-up)
  • Hands on training

  • TMT labeling
  • Data analysis for qualitative proteomics
  • Hands on training

  • Data analysis for quantitative proteomics

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