AGRI
BIOTECH FOUNDATION
Rajendranagar,
Hyderabad-500030
RESEARCH
PROJECTS on “Agri Biotechnologies”
for
B.Sc. / B.Tech / M.Sc. students
Applications
are invited for theme based project/dissertation works on Plant molecular
biology (Genetic engineering and Molecular Marker assisted breeding), plant
tissue culture and Microbial biotechnology
Agri Biotech
Foundation is an autonomous Institution engaged in research, extension and
training. It is located on Prof. Jayashankar Telangana State Agriculture University
Campus, Rajendranagar, Hyderabad. ABF has been recognized as Scientific and
Industrial Research Organization (SIRO) by the Department of Scientific and
Industrial Research (DSIR), New Delhi.
Themes of dissertations: Dissertations
can be taken up on the following themes
Theme 1. Genetic engineering:
Gene Cloning: Isolation of
gene, promoter, Restriction analysis, ligation, cloning in different vectors
(TOPO-TA, plant expression vectors), DH5α transformation, PCR analysis
Agrobacterium mediated plant
transformation:
Mobilization of plant expression vectors into Agrobacterium by freeze
thaw method and electroporation, explant preparation, co-cultivation,
differentiation of transformed and non transformed explants, optimization of
regeneration of shoots and roots in different hormonal growth media,
orientation on plant growth conditions at glass house.
Characterization of biotechnologically
developed plants
PCR analysis for identification of
transformed and non transformed plants, Southern blot analysis for detection of
copy number of transgenes, training on different stress impositions on plants,
sample collection, Biochemical and phenotypic characterization, protein
detection through strip assays.
Gene expression studies: RNA isolation,
cDNA synthesis, quantitative transcript analysis of gene
Theme 2. Marker assisted breeding
Tagging and
mapping gene(s) of interest in rice/ blackgram
Development of
mapping populations
Phenotyping
for resistance to bacterial blight, blast, BPH, WBPH or gall midge
Genotyping
with SSR, EST-SSR markers
Linkage
analysis and QTL mapping
Identification
and functional validation of candidate gene
Theme 3. Plant Tissue culture
Standardization of regeneration protocols
for various species through organogenesis and somatic embryogenesis.
Techniques to be covered: Nutrient media
preparation, various sterilization methods, Explants isolation and inoculation,
multiplication through sub culturing, in vitro & in vivo rooting
and acclimatization.
Theme 4. Microbial biotechnology
Genomic DNA from
Gram+ve, Gram–ve bacteria and fungi;
Bar coding in
microbial genomics;
Cloning and transformations
techniques in Plant-micro interactions;
Fluorescence image sensing techniques;
Transcriptomic studies in
plant-microbes;
Bacterial
protein profile, quantitative and qualitative analysis-SDS and Western
blotting; Plant probiotics,
Piolet scale fermentation techniques
Chromatographic techniques (Column, TLC,
HPLC)
Course
fee:
It is based on duration of the project
Eligibility: B.Sc., B.Tech. M.Sc.,
any life sciences
Web site: for more details visit our
website: www.abfindia.org
Contact details:
Interested persons may contact Dr. G.
Mallikarjuna, Assistant Professor-Plant Molecular Biology, Agri Biotech
Foundation, Hyderabad-500030
garladinnemarjun@gmail.com; Mobile:
91-8142799656;
Office: 040-29803417/29807011
Filled in application should be sent to:
‘The Executive Director, Agri Biotech Foundation, PJTS Agriculture University
Campus, Rajendranagar, Hyderabad-500030”
Agri Biotech Foundation,
Hyderabad-500030
Research
projects on “Agri Biotechnologies” for B.Sc. / B.Tech / M.Sc. students
1.
|
Full name (in capital letters)
2.
Date of Birth
3.
Sex
4.
Present Position
5.
Department
6.
Address
7.
Contact
Telephone Number/Mobile:
(with STD code)
8.
E-mail ID :
9.
Educational Qualifications
a.
B.Sc/B.Tech
b.
M.Sc
c.
Other
10. University/College
and Subjects
11. Theme
of your project/Title of the project:
12. Dates
and Duration of the project:
13. Reasons
for choosing the dissertation work:
14. Any
other details you want to give
Signature of the Candidate
Date and Place:
Send the completed
Applications to: The Executive Director, Agri Biotech
Foundation, PJTS Agriculture University Campus, Rajendranagar, Hyderabad 500
030, Telangana (or) Send soft copies to garladinnemarjun@gmail.com
0 Comments