One Month International Comprehensive bioinformatics Workshop on - Transcriptomics with NGS by Decode Life

Registration is open for one month Bioinformatics Workshop by DE<code>LIFE

Duration : 6 September - 29 September, 2025

Registration Link - https://decodelife.co.in/

Fees: Rupees 1300 for Indian Participants /USD 30 for International Participants.

Timing : 7. 00 PM Indian standard time.

Schedule

Serial number	Date	Day	Time	Topic
1	September 6, 2025	Saturday	7. 00 PM IST	Introduction to Command Line
2	September 7, 2025	Sunday	7. 00 PM IST	Advanced Linux/Unix Commands
3	September 8, 2025	Monday	7. 30 PM IST	Core Skill: R programming
4	September 9, 2025	Tuesday	7. 30 PM IST	Core Skill: R programming
5	September 10, 2025	Wednesday	7. 30 PM IST	Core Skill: R programming
6	September 11, 2025	Thursday	7. 00 PM IST	Raw data QC Trimming strategies References & indices Alignment Counting & import , Normalization
7	September 12, 2025	Friday	7. 00 PM IST	Differential Expression (DE), Volcano plots; heatmaps; storytelling
8	September 13, 2025	Saturday	7. 00 PM IST	Small RNA/miRNA-seq
9	September 14, 2025	Sunday	7. 00 PM IST	Small RNA/miRNA-seq miRDeep2; QC of length profiles; differential miRNA lncRNA & circRNA Annotation issues; CIRI2; lncRNA functional hypotheses
10	September 15, 2025	Monday	7. 00 PM IST	RNA fusions & variants detection using NGS datasets
11	September 16, 2025	Tuesday	7. 00 PM IST	Isoforms & splicing
12	September 17, 2025	Wednesday	7. 00 PM IST	Batch effects & confounders
13	September 18, 2025	Thursday	7. 00 PM IST	Fundamentals of Single-Cell Genomics Overview of single-cell analysis and its importance. Comparison with bulk sequencing. Key technologies and workflows (scRNA-seq, scATAC-seq).
14	September 19, 2025	Friday	7. 00 PM IST	Quality Control and Preprocessing Filtering low-quality cells and genes. Normalization techniques (log-normalization, SCTransform). Batch effect correction (Seurat, CCA, Harmony).
15	September 20, 2025	Saturday	7. 00 PM IST	No session -OFF
16	September 21, 2025	Sunday	7. 00 PM IST	No session -OFF
17	September 22, 2025	Monday	7. 00 PM IST	Dimensionality Reduction and Clustering PCA, t-SNE, UMAP for data visualization. Clustering and Cell Type Identification Clustering methods (Louvain, Leiden, K-means, HDBSCAN, SC3).
18	September 23, 2025	Tuesday	7. 00 PM IST	Cell Type Annotation Marker gene identification. Reference-based and de novo annotation methods.
19	September 24, 2025	Wednesday	7. 00 PM IST	Differential Expression and Pathway Analysis Differential Gene Expression (DGE) Analysis Identifying cluster-specific and condition-specific genes. Pseudobulk analysis.
20	September 25, 2025	Thursday	7. 00 PM IST	Single-Cell RNA Velocity and Time-Series Analysis Predicting future cell states using scVelo. Mapping developmental transitions. Advanced time-series modeling for single-cell data.
21	September 26, 2025	Friday	7. 00 PM IST	Trajectory and Pseudotime Analysis Reconstructing cell differentiation pathways (Monocle). Identifying branching and decision points
22	September 27, 2025	Saturday	7. 00 PM IST	Cell-Cell Communication Analysis Intercellular crosstalk analysis. Practical session using CellChat in R.
23	September 28, 2025	Sunday	7. 00 PM IST	scATAC+scRNA integration;
24	September 29, 2025	Monday	7. 00 PM IST	Spatial transcriptomics Visium/Slide-seq; spatial QC; clustering