Indian Institute of Science (IISC)
Bangalore 560 012
Teacher/Researcher Training Workshop in Biological Sciences 2010:
Applications from teachers and researchers belonging to universities, colleges and affiliated institutions are invited to the Second 2010 Workshop in Biological Sciences being held during November 22-29, 2010 at the Indian Institute of Science.
The workshop covers theory classes in basic and contemporary topics in various areas of biological sciences and hands-on training on various techniques in protein purification.
Applicants should send their brief data including a paragraph on the current research they are involved in as well as a list of publication, if any to the coordinator of the UGC Networking Centre. Since the number of vacancies is limited, preference will be given to those who have completed their M.Sc. degree and are currently engaged in teaching and research and are already or will be employed by their universities.
Boarding and lodging will be arranged by the institute for the duration of the workshop. Travel expenses by train will be reimbursed according to institute guidelines.
For more information, you are advised to visit the UGC Networking Centre Website at http://mcbl.iisc.ernet.in/UGC/. You may also contact us at the address UGC-NRC-DBS, Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, and send application to the Coordinator at the e-mail ugc-nrc-dbs@mcbl.iisc.ernet.in. The last date for receiving applications is September 20, 2010.
ABOUT THIS COURSE:
‘Techniques in Protein Purification: November 22- November 29, 2010
The study of proteins/enzymes and their function is central to understanding both cells and organisms. Proteins serve as catalysts that maintain metabolic processes in the cell, as structural elements both inside and outside the cells and as signaling molecules between cells and their components. Understanding the properties of proteins and determination of protein concentration is an essential technique in all aspects of protein and proteomic studies. Proteins differ in size, shape, isoelectric properties, hydrophobicity and biological affinity. Study of these properties is necessary for understanding their structural and functional roles and their interrelationship in living organisms.
Course Instructors
1. Prof. Siddhartha Sarma, Molecular Biophysics Unit
2. Prof. Rajan Dighe, Department of Molecular Reproduction, Development & Genetics
3. Prof. B. Gopal, Molecular Biophysics Unit
4. Prof. D.N. Rao, Department of Biochemistry
Laboratory Exercises
1. Assays for Protein Quantification
2. Protein electrophoresis-SDS-PAGE/Native PAGE
3. Sub Cellular Fractionation
4. Ammonium Sulphate Fractionation
5. Dialysis/Gel Filtration
6. Ion Exchange Chromatography
7. Molecular Exclusion Chromatography (Molecular Weight determination)
8. Affinity Chromatography
9. Western Blotting
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