ANNAMALAI UNIVERSITY DBT & CSIR SPONSORED National Workshop on Plant-Biotechnology - RAPD-PCR Techniques 2011

DBT & CSIR SPONSORED

National Workshop on Plant-Biotechnology - RAPD-PCR techniques 

August, 18th – 20th, 2011

Organized by

DEPARTMENT OF BOTANY
(DST-FIST & UGC-SAP Sponsored Department)
ANNAMALAI UNIVERSITY
ANNAMALAI NAGAR – 608 002 

TAMIL NADU 

HOST DEPARTMENT

The Department of Botany has an Outstanding Record of promoting Excellent Research, right from its inception. It has been recognized as DST-FIST & UGC-SAP Sponsored Department. The Department offers 2 year M.Sc. Botany and 5 year integrated M.Sc. Herbal Science Programme. In addition, M. Phil. and Ph.D. programs are offered. The Department has produced 161 Ph.Ds in a variety of botanical disciplines such as Biodiversity and its Conservation, Genetics, Cell and Molecular Biology, Microorganisms and Plant Pathology, Pollution and Environmental impacts on Ecosystem, Plant Physiology and Biotechnology, Plant Taxonomy and Plant Genomics. The Department has organized workshops, seminars and refresher courses through which a huge number of participants from various universities and institutes took part and have placed benefits. 

WORKSHOP BACKGROUND 

The Polymerase Chain Reaction (PCR) is a technique that permits geometric and enzymatic amplification of specific sequences of DNA. The PCR is now widely used in research laboratories and doctor's offices, relies on the ability of DNA-coping enzymes to remain stable at high temperatures. No problem for Thermus aquaticus, the sultry bacterium from yelllowstone that now helps scientist to produce million of copies of a single DNA segment in matter of hours. In nature, most organisms copy their DNA in the same way. The PCR mimics this process, only in test tubes. When any cell divides, enzymes called polymerase made a copy of the entire DNA in each chromosome. Besides, based on the applications, PCR has been modified in a number of ways to suit specific application situation, several molecular markers (RFLP, AFLP, ISSR, SSR, SNP and RAPD) also available for analyzing genetic variations at molecular level. Among these, RAPD is an inexpensive and a rapid method not requiring much information regarding the genome and has been widely used to document the genetic diversity in plants and animals. Marker-based genome analyses have become increasingly important for various fields of plant science research. In this background, the present workshop will help to provide researchers a hands-on training in molecular marker techniques and to highlight the importance of marker assisted techniques in the plant biotechnology research. 

PROGRAMME SCHEDULE 

Morning Session      : 9.00 am to 1.00 pm;

Lunch                     : 1.00 pm to 2.00 pm

Afternoon Session   : 2.00 pm to 6.30 pm

Tea break in the middle of each session (15min.) 

WORKSHOP PROGRAMME 

Besides hands-on training, participants will be encouraged to carry out the techniques themselves. The workshop will be conducted for 30 participants only. This workshop of RAPD-PCR technique will cover guest lectures and hands-on experience on various aspects of biotechnology as follows 

Laboratory Safety

Tools

Knowledge-information regarding chemicals and other basic instruments required

Major instruments – PCR-thermal cycler, Electrophoresis, Gel documentation.

Chemicals preparation for

DNA isolation and purification.

DNA quantification

Agarose-gel Electrophoresis

PCR reactions

Techniques to be handled

DNA extraction

Isolation and purification of genomic DNA (plant sample).

DNA quantification in spectrophotometer

DNA qualitative analysis in agarose-gel electrophoresis

Amplification in PCR thermal cycler

Initial denaturation, Denaturation, Annealing and Extension.

Agarose-gel Electrophoresis

Gel-documentation

Data scoring

Data analysis in software

Results and interpretation

REGISTRATION 

The participants are requested to fill-in the Registration form and mail it to the Organizing Secretary along with a demand draft for Rs. 2000/- from Indian Bank / ICICI Bank / SBI in favour of "The Registrar, Annamalai University" payable at Annamalainagar or Chidambaram. Only 30 participants can be accommodated. The first registering persons would be preferred. 

VENUE: Department of Botany, Annamalai University, Annamalainagar-608 002, Tamilnadu.

ACCOMMODATION: The external participants can be given accommodation for 3days in the university guest house/hostel on request.

LAST DATE: 

Last date to receive the filled-in application: 1st August, 2011. Upon receipt of the completed application form, the confirmation of registration will be intimated to the participants via email.

For correspondence 

     Dr. R. SELVARAJ
       Organizing Secretary
       Professor
       Department of Botany
       Annamalai University
       Annamalainagar – 608 002
       Tamilnadu

       Tel: Off : (04144)-238282  Ext.354  Fax 238080
       Res: (04144)-239014
       Email: selvarajphd14@yahoo.co.in
       Mobile: 94429 09910

REGISTRATION FORM - Download