DBT Sponsored
Workshop on Microbial Enzyme Technology
Date: 16th to 19th May 2012
Organised by
Microbiology Programme,
Dept. of Biochemistry and Molecular Biology
School of Life Sciences
Pondicherry University, R.Venkataraman Nagar, Kalapet, Puducherry-605014
Training Programme
Enzymes have long been used as alternatives to chemicals to improve the efficiency and cost-effectiveness of a wide range of industrial systems and processes. They are currently used in basic and applied arenas of research as well as in a wide range of product design and manufacturing processes, such as those pertaining to the food, beverage, pharmaceutical, detergent, leather processing, and peptide synthesis industries. Among these biocatalysts, high-alkaline proteases, which alone account for about 40% of the total worldwide enzyme sales.
Two types of microbial enzymes (extracellular and intracellular) are industrially important. Screening of both types of enzymes from different environments sewage, marine waste, sea sediments and garbage waste and contaminated food stuffs) will provide enzyme characteristic and its activity on different environmental conditions.
This workshop will deal with the isolation and characterization of microbial enzymes.
Objectives
Screening of Microbial Enzymes
Enzyme activity - Protease, Invertase
Enzyme Extraction- Intracellular and Extracellular
Enzyme Purification Techniques (Column chromatography)
Molecular weight determination for enzymes
Enzyme kinetics (Michaelis-Menten Equation)
Immobilization of enzymes for packed bed reactor analysis
Theoretical aspects of MALDI TOF MS (Protein sequence)
The laboratory sessions will follow a lecture on a recent topic in enzyme technology
Who can participate?
The intention of this workshop is to propel the Students, Research Scholars, Faculty
members from CollegelUniversity, Young Scientist from Research Institutions and
Companies to acquire knowledge in Enzymology. All the participants can have hands on training in these techniques.
Registration Fee
Academic /industry Staff - Rs. 800/Research
Scholars/ Students - Rs 400/The
registration fees include workshop materials, working lunch and refreshments.
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