T. R. Prabha, K. Revathi, M. S. Vinod, S. P. Shanthakumar and Paul Bernard
Water moulds grow on submerged and floating leaves, twigs and wood in well-aerated waters. The morphological descriptions of many rare fungal species have been increasingly supplemented by their molecular characteristics. The isolation of genomic DNA is a prerequisite for their molecular characterization. The DNA isolation procedure developed herein is based on the sodium dodecyl sulphate/phenol method, without the addition of β-mercaptoethanol and proteinase K; instead it uses phenol/chloroform extraction. This protocol resulted in good quality DNA which was further validated using polymerase chain reaction amplification, cloning and sequencing and other downstream processes. The whole procedure can be done in a relatively short period of time without the need to employ β-mercaptoethanol, CTAB, lysozyme digestion, proteinase K treatment, etc., thereby reducing the overall costs involved.
Full Article : http://cs-test.ias.ac.in/cs/Volumes/104/03/0345.pdf
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