for CSIR | Heat shock at 37 ° C with plasmid ligated at 37 ° C yields more number of Escherichia coli transformants than plasmid ligated at 16 ° C: a possible role of ligated plasmid co nformation during heat shock | Current Science Article

Anjan Barman, Praveen Kumar, Vinay Kumar, Robin Doley and Suvendra Kumar Ray

The phenomenon of temperature effects on plasmid conformation has been well elucidated. However, the impact of change in plasmid conformation caused by temperature shift (during the heat shock mode) on transformation outcomes has not been reported till now. Here, we analyse transformation efficacy at 37°C of plasmid pBSKS ligated at three different temperatures (4° C, 16° C and 37° C) to Escherichia coli DH5α strain. Though ligation was effective at all the three temperatures mentioned above, the number of trans-formants as more with plasmid DNA ligated at 37° C than at 4° C and 16° C. Our study indicates a possible role of plasmid conformation on the effect of heat shock transformation.

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