OSMANIA UNIVERSITY
FLOWCYTOMETRY SOLUTIONS Pvt. Ltd.
Organizes
Hands on Flow Cytometry Workshop on Basics and Immunophenotyping
March 10-12, 2014, Hyderabad
About DBT OU ISLARE
Department of Biotechnology, Government of India, funded Osmania University to set up an inter-disciplinary School of Life Science for Advanced Research and Education. The main objective of this program is to expose and train budding Life Science students and research scholars with advanced and state of the art technique enabling them to carry out research in frontier area of Science and Technology. As a part of this program we are planning to organize a series of workshops to train students in advance techniques.
The present workshop on flow cytometry exposes students with basics of this technique and hands on experience in exploring the instrument for their research needs.
About Flowcytometry Solutions (FlowSols) Flowcytometry Solutions (FlowSols) is an organization, which aims at providing complete solution to the researchers and clinicians on flow cytometry. We offer to provide support to the students and researchers engrossed to flow cytometry pitch, towards addressing problems pertaining to instrument handling, experiment design and applications, sample preparation, acquisition, data investigation and presentation. Our mission is to provide a complete flow cytometry expertise, which can help researchers to innovate, lead and excel (www.flowsols.com).
PROGRAM
Day 1:
09.30-09.45: Welcome note & Scope of the workshop
09.45-11.00: Introduction to Flow Cytometry and its Applications
11.00-11.15: Tea Break
11.15-12.15: Anatomy of a Flow Cytometer and monitoring its health (Quality control).
12.15-13.15: Concepts of Multicolor Flow Cytometry; Fluorochromes, Spectral Overlap, Compensation Controls standards, Phenotyping and Cell Surface Staining
13.15-14.00: Lunch
14.00-15.00: Instrument startup and demonstration of instrument setup; Template preparation for data acquisition, PMT Voltage setting, Threshold etc.
15.00-17.30: Wet Lab: Cell Cycle Module: Cell cycle analysis using PI (Cell cycle data analysis, Doublet discrimination etc.)
Day 2:
09.30-10.00: Review of Day 1 and queries
10.00-11.00: How to set up and Design a Multicolor Flow Cytometry Experiment
11.00-11.15: Tea Break
11.15-13.00: Concepts of Compensation for multicolor flow cytometry.
13.00-14.00: Lunch
14.00-17.30: Wet Lab: Immunophenotyping: Staining of the cells (Whole Blood profiling) for multicolor flow cytometry (4 colors), and preparation of Compensation controls. (This will cover usage of antibodies and identification of the % of different cell populations, phenotyping and cell surface staining). Sample acquisition, data analysis and discussion.
Day 3:
09.00-09.30: Review of Day 2 and queries
09.30-11.00: Wet Lab: Immunophenotyping (contd.)
11.00-11.15: Tea Break
11.15-13.00: Wet Lab: Immunophenotyping (contd.)
13.00-14.00: Lunch
14.00-16.30: Concepts and Basics of Flow Data Analysis—What to do and what not to do? Analysis of flow cytometry data and reporting results.
1630-1700: Flow cytometry troubleshooting and discussion and Q&A. Test, evaluation and feedback
1700: Valedictory session
FACULTY: VENUE
Dr. Hemant Agrawal DBT-OU-ISLARE
Director—Flowcytometry Solutions Pvt Ltd. Central Facilities for Research and Development
flowsols@gmail.com Osmania University
+91-7665130114 Hyderabad-500007
Ph: 040-27682469/65742392
Prof. S. Satyanarayana Singh, Coordinator
DBT-OU-ISLARE Central Facilities for research and development
Osmania University
Hyderabad-500007
For more details please contact:
Ph: 040-27682469/65742392
dbtouislare@gmail.com
* Complete application with registration fee should reach on or before March 6th, 2014
Brochure: www.osmania.ac.in/EventsConf2014/Cytometry%20workshop%20flier_March%202014_revised_04feb2014.pdf
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