KIIT-TBI Bioincubator Summer Training Program 2014

Summer Training Programme

KIIT-TBI Bioincubator organizes summer training programme each year to enhance hands on practical knowledge of life sciences students. This programme is designed to provide Masters and PhD students with an exposure to an international quality biotechnology research environment. A service fee is charged from trainees to cover the costs of the training*. The fee structure and duration of training programme is given below:

Duration Fee

1 month 7000
45 days 10000

Training Modules:

Industrial Microbiology

 Basic Microbiological techniques
 Isolation and Culturing of microbes
 Biochemical and Molecular Characterisation of microorganisms
 Batch fermentation in flasks and subsequent Scale up to Fermenters
 Fermentation (Submerged, Gaseous and Solid State)
 Downstream processing
 Purification , Characterization and Immobilization
 Quality analysis in Bio-industries

Recombinant DNA Technology

 Plasmid Isolation
 DNA isolation
 Agarose Gel Electrophoresis
 Qualitative & Quantitative analysis of Nucleic Acid
 Polymerase Chain Reaction (PCR)
 Restriction Digestion
 Gel Elution
 Competent Cell Preparation
 Transformation
 Primer Designing and Gene Cloning
 Screening of Positive Clones
 Real Time PCR

Protein Biochemistry

 Protein Overexpression and Purification
 Chromatography Techniques (Ion-exchange, Affinity Chromatography)
 Estimation of Protein by Bradford, BCA and Lowry’s Method.
 Characterisation of Proteins through SDS-PAGE
 Silver Staining
 Western Blotting
 ELISA

Infection Biology and Immunology

 Cell Invasion assay
 Macrophage replication Assay
 GFP Reporter Assay
 Cytokine analysis
 ELISA
 Flow Cytometry
 Extraction and purification of IgG from plasma/serum
 Preparation of Antigens for immunisation

Bioinformatics

 Sequence Alignment (Pairwise Alignment, Database search, Multiple Alignment).
 Phylogenetic Analysis
 Docking Analysis
 Structural Analysis
 Network Analysis
 Gene Prediction
 Motifs and Domains
 Databases
* Food and Accommodation is not provided by the institute.

Last date : 30th April 2014

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