SRM Workshops on TA cloning and Cytogenetics

Department of Genetic Engineering

SRM University, Kattankulathur-603203

“A ONE DAY Workshop on METHODS AND MECHANISMs OF HUMAN Cytogenetics”

May 18th, 2015

Organized by

Department of Genetic Engineering,

SRM University

ABOUT THE WORKSHOP: The main goal of the workshop is to familiarize the participants with the cytogenetic techniques.

OBJECTIVES OF THE PROGRAM: The workshop involves lectures and hands on training sessions about various cytogenetic parameters. Chromosome mutations and related events are the cause of many human genetic diseases and there is substantial evidence that chromosome mutations and related events causing alterations in oncogenes and tumour suppressor genes are involved in cancer in humans and experimental systems. The human chromosome analysis is a key tool for research and progress in clinical management of human genetic diseases including cancer, mainly leukaemia. With the advent of molecular cytogenetics and DNA based techniques, the conventional method of chromosome analysis is thought to be on way to extinction, however, till today, the human chromosome preparation and GTG-banded karyotyping has evolved to Molecular Karyotyping but, is not replaced. Majority of the students are not aware about cytogenetic methods.

Therefore it will help them to experience hands-on practical training,

OUTLINE OF THE CONTENTS: 

i. Hands-on experiment will be done by the participants to obtain metaphase chromosomes using bone marrow cells of Swiss albino mice.

ii. Experiment will be done by the participants to obtain metaphase chromosomes using short term cultures of peripheral blood lymphocytes of healthy human beings. Mitogen stimulated blood culture, harvesting, slide preparation, staining, and microscopy will be performed. Methods and mechanisms will be explained with current updates on applied aspects of the field of conventional cytogenetics.

iii. Chorio allantoic membrane assay in chick embryo

Venue: Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Chennai-603203

Workshop Timing: 9.30 a.m. to 5.00 p.m.

Registration Details: The registration fee for the workshop is Rs.500/- per participant.

Registration fee includes:

• Participation in the workshop

• Lunch will be provided

• Certificate for successful completion.

• Transportation is not included in the registration fee. Participants will have to

arrange for their own transport.

Eligibility: Life Science Students (Under graduate) from Colleges.

Mode of payment: DD/Cheque

Last date for registration: 15^th May, 2015

Bank details:

Name- P. Surya teja

Account number-117001001832812

Branch-City Union Bank Ltd, SRM Branch, Kattankulattur.

Number of Participants: The total number of participants is limited to 30 on first cum first basis.

For details contact:

Dr. Rashmi Mohapatra Assistant Professor Department of Genetic engineering SRM University Mobile Number: 9840537631 Email: rashmimohapatra.i@ktr.srmuniv.ac.in

Dr. A.Swapna Geetanjali Assistant Professor Department of Genetic engineering SRM University Mobile Number: 9789814798 Email: swapnageetanjali.a@ktr.srmuniv.ac.in

“Workshop and Hands on Training on One Step TA Cloning”

May 19th – 20th, 2015

Organized by

Department of Genetic Engineering,

SRM University

About the Workshop: The main goal of the workshop is to familiarize the participants with the advances in Gene cloning techniques.

Workshop Topics: The two days workshop involves lectures and hands on training sessions about Gene cloning in TA vector. Different topics for example, Gene cloning, restriction and digestion, Update on Cloning strategies, Hands-on experience on TA cloning will be covered in the workshop.

Venue: Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Chennai-603203

Workshop Timing: 9.30 a.m. to 5.00 p.m.

Registration Details: The registration fee for the workshop is Rs.500/- per participant.

Registration fee includes:

• Participation in the workshop
• Lunch and Tea/coffee breaks for all two days of the workshop
• Certificate for successful completion.
• Accommodation is not included in the registration fee. Participants will have to arrange for their
own accommodation.

Eligibility: Life science Students (Under and Post graduates) B.Sc, B.tech, M.Sc & M.tech from Universities, Engineering Colleges and Arts and science colleges.


Number of Participants: The total number of participants is limited to 30 on first cum first basis.

Eligibility: Life Science Students (Under graduate) from Colleges.


Mode of payment: DD/Cheque


Last date for registration: 15^th May, 2015

Bank details:

Name- Swapna Geetanjali. A
Account number-260001001890780
Branch-City Union Bank Ltd, Ambattur Branch, Chennai-600053.


For details contact:

Organisers:

Dr. A.Swapna Geetanjali Assistant Professor Department of Genetic engineering SRM University Mobile Number: 9789814798 Email: swapnageetanjali.a@ktr.srmuniv.ac.in

Dr. Rashmi Mohapatra Assistant Professor Department of Genetic engineering SRM University Mobile Number: 9840537631 Email: rashmimohapatra.i@ktr.srmuniv.ac.in

Programme

Day-1 (28-4-2014/9:30 am to 5:00 pm)

9:30-10:30-Registration
10:30-12:30- PCR/Cloning methodology presentation
12:30-1:30-Agarose gel electrophoresis/Lunch Break
1:30-2:30- Gel purification (Gel elution)
2:30-5:00-Ligation and Transformation

Day-2 (29-4-2014/9:30 am to 3:00 pm)

9:30-12:30-Colony PCR/Participants will be taken to department lab visits
12:30-1:30-Agarose gel electrophoresis/Lunch Break
2:00-3:00-Valedictory function (Distribution of certificates)