National Level Workshop in NGS Data Analysis @ RASA LSI​

Next-Generation Sequencing Data Analysis Workshop: An overview

           Our 3 days and 4 days hands-on NGS Data analysis workshop will provide an detailed overview to data analyses, tools and resources for genomics and transcriptomics next generation sequence data. It will be delivered using a mixture of lectures, computer-based practical sessions and discussions with case studies from life science research. This workshop will also introduce the Galaxy platform and walk participants through a multi-step next generation sequencing (NGS) data analysis, starting with quality control. We will review common choices in NGS data analysis, and demonstrate them within the context of Galaxy, taking full advantage of Galaxy's rich tool set and visualization capabilities. We will also provide a brief overview of what is needed to set up your own local Galaxy instance. Galaxy is an open-source, NIH-funded project.

Practical Application:

·       Understand the Next generation sequencing
·       Discuss the advantages and limitations of the sequencing technologies
·       Learn how to use Galaxy server to analyze the NGS data
·       Deal with the raw Illumina data with quality evaluation and filtering
·       Map reads to the reference genomes, call SNPs and predict their phenotype consequence
·       Alignment to a reference, SNP and structural variant-calling of next generation DNA and RNA data
·       Use some bioinformatics software and tools to aid with the analysis of NGS data
·       Understanding Gene expression and Differential gene expression
·       Performing the diversity analysis using metagenomic tools
·       Overview on ChiP-Seq analysis (Day 4)
·       Visualize your data in genome browsers and make conclusions
·       Participants will receive workshop training certificate.

Topic to be covered:
  • Introduction to sequencing technologies from a data analysts view
  • Common NGS data analysis issues
  • Applications of sequencing technologies
  • Notations and NGS Terminolgy
  • Introduction to the command line and important commands- Linux
  • Introduction to bioinformatics file formats (e.g. FASTA, BED) and databases (e.g. UCSC)
  • Raw sequence files (FASTQ format)
  • Preprocessing of raw reads: quality control (FastQC), adapter clipping, quality trimming
  • Introduction to read mapping (Alignment methods, Mapping heuristics)
  • Read mapping (BWA, Bowtie2, STAR, segemehl)
  • Mapping output (SAM/BAM format)
  • Usage of important NGS toolkits (samtools, BEDtools)
  • Introduction to DNA-seq analysis with Galaxy
  • Introduction to RNA-seq analysis with Galaxy
  • Performing diversity analysis of the metagenome
Day 4
  • Introduction to ChIP-seq analysis with Galaxy
Databases and Tools & Software’s used
·    SRA, GEO Database, NCBI, EMBL, Galaxy, wAANOVAR, FREEBAYES, BOWTIE, BWA, Varscan, HTcount, DESEQ2, MACs, IGV, UCSC genome browser and many more.

Eligibility: Working professional,Scientists /Research fellows (National Laboratories, Universities & other R&D institutions) and Graduate / Post graduate students in Engineering / Biological / Chemical sciences/ Medical discipline.

Workshop Date – 14th Dec – 17th Dec 2017

 Fees: For Registration

Dates for Registration
Professor/ Researcher
3 Days
4 Days
3 Days
4 Days
3 Days
4 Days
Early Bird
Till 14 Nov 2017
15th Nov- 13th Dec 2017
·         *Includes lunch, & snacks.
·         **Id proof required at the time of registration.
·         ***Fees are non transferrable & non refundable in any case.


Mr.Sameer Chaudhary 

Sr.Scientist, RASA Life Science Informatics.

Tanvee Pardeshi

Application Scientist, RASA Life Science Informatics.

Sakshi Rawat

Jr. Application Scientist, RASA Life Science Informatics.

Websites: | /company/rasa-life-science-informatics,

Venue: RASA Life Science Informatics
4th floor.46/10 shakuntal,
Law college Road,Erandwane 411004


Bank Name & Branch: IDBI, F.C. Road, Pune.


A/C Number: 007102000025762

IFSC Code: IBKL0000007

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