Four Week Skill Development Course
June 1, 2018 to June 30, 2018
School of Agricultural Biotechnology
Punjab Agricultural University
Ludhiana
Email: dirsoab@pau.edu
Plant Tissue Culture (PTC)
Week 1 Orientation to PTC facility and Media preparation and sterilization
• Good laboratory practices and safety measures
• Orientation to plant tissue culture facility and protected structures
• Nutritional requirements of in vitro cultures, stocks and media preparation
• Preparation of stocks and culture media
Week 2 Stages in micropropagation
• Mother plant selection, collection and preparation
• Aseptic culture techniques for establishment and maintenance of cultures
• Callus culture from leaves
• Micropropagation of sugarcane
• Micropropagation of mentha and stevia
Week 3 Rooting, hardening and plantation
• Subculturing of Sugarcane, Mentha and Stevia
• Rooting of the plant tissue cultures
• Hardening of the rooted plantlets
• Transfer to the soil and maintenance of the plants
Week 4 Management, marketing and certification in PTC
• Economics of establishing a commercial plant micropropagation unit
• Certification of micropropagated plants
Molecular Marker Technology
Week 1 Conventional and High throughput DNA extraction procedures
• Large scale DNA extraction using CTAB method.
• High throughput DNA extraction for large number of samples
• Gel based and high throughput procedures for DNA quantification
Week 2 Insights into DNA
• Concepts of polymerase chain reaction
• Molecular marker and primers; concept development and design
• Conventional PCR and its varinats: familiarization and setup
Week 3 PCR product analysis
• Preparation of Agarose and PAGE gels
• Visualization and analyzing PCR product
• SSR markers and their development
• Germplasm characterisation and testing purity of hybrids using SSR markers
Week 4 Gene mapping and Marker assisted selection
• Introduction to gene and genetic mapping
• Mapping populations and their development
• Different approaches for mapping genes
• Development of linkage maps and mapping genes of interest
• Concept of MAS and its applications
Bioinformatics
Week 1 Basic Bioinformatics concepts
• Concepts of computing and Operating system (Window, Linux)
• Introduction and overview of Bioinformatics
• Biological Databases and sequence file formats
• Pairwise and multiple sequence alignment
• Phylogenetics tree construction methods
Week 2 Gene Prediction methods
• Introduction to gene prediction methods
• Prokaryote gene prediction methods
• Eukaryote gene prediction methods
• Gene annotation methods
• Non-coding DNA
Week 3 Protein Structure
• Amino acids, protein structure classification and prediction methods
• Secondary structure prediction methods
• Tertiary structure prediction methods
• Structure evaluation and refinement techniques
• Docking studies of proteins
Week 4 Genomics studies
• SSR identification and primer designing
• SNP identification and designing of primers
• miRNA identification
• miRNA based SSR design
• Discussions and Feedback
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