DNA melting temperature (Tm) was found to be 47°C and enthalpy measured at Tm was 0.032 kJ. The entropy change would be:

Entropy = Enthalpy/Tm

Enthalpy = 0.032 kJ = 32 J
Tm = 47 C or 320 K

Entropy = 32/320 = 0.1 J

= 0.0001 kJ

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