Centre for Ayurveda Biology and Holistic Nutrition (CABHN)
The
University
of
Trans-Disciplinary Health Sciences and Technology (TDU)
74/2, Jarakabande Kaval, Post Attur, via Yelahanka
Bengaluru - 560064
Position: JRF (1 No.)
Project: Multi-omic approaches and mass spectrometry based tissue metabolite imaging towards understanding metabolites localisation and metabolic pathways in specific cell/tissues of key medicinal plant, Dysoxylum binectariferum.
Funding Agency: SERB, DST, Govt. of India
Duration: 3 years
Fellowship: As per SERB, DST norms
Requirement: immediately
Description: Advanced multi-omics technologies provide revolutionary strategies to study complex biological processes, e.g. to systematically decipher biosynthetic pathways to mechanistically challenging, structurally complex, widely used plant medicinals that cannot be economically synthesized. A multi-omics strategy is proposed to deduce biosynthetic pathway to rohitukine, a chromone alkaloid, widely used in cancer treatment. Distinct cell types involved in plantawill be identified using mass spectrometry based metabolite imaging in situand laser microscope dissection. Knowledge gained can be applied to developing synthetic biology approaches in order to obtain new sources of these compounds in other host systems.
Skills sets required
· Molecular and biochemical techniques
· Isolation, purification and structural characterization of secondary metabolites
· Experience in Chromatographic techniques like HPLC, GCMS and LCMS
· Experience in RNA isolation, Synthesis of cDNA. PCR, RT-PCR, qPCR, cloning and transformation.
· Bioinformatics: transcriptome analysis
References
·
Mohana Kumara P, Srimany A, Arunan S, Ravikanth G, Uma Shaanker R, Pradeep T. 2016Desorption Electrospray Ionization (DESI) Mass Spectrometric Imaging of the Distribution of Rohitukine in the Seedling of Dysoxylum binectariferumHook. F. PLoS ONE 11(6): e0158099. doi:10.1371/journal.pone.0158099IF: 3.54
· Mohana Kumara P, AmitavaSrimany ,Ravikanth G , Uma Shaanker, R. Pradeep, T.2015Ambient ionization mass spectrometry imaging of rohitukine, a chromone anti-cancer alkaloid, during seed development in Dysoxylum binectariferumHook. F (Meliaceae). Phytochemistry. Volume 116, 104-110. IF: 3.35
see this link for details: https://mohanmultiomics.wordpress.com/
To apply: Send out the detailed CV. All selected candidates will be informed. No TA/DA will be provided. Interested candidates to send their cv to mohan.kumar@tdu.edu.in and cc to hr@tdu.edu.in
Start date: July 2019.
Duration: One-year contract, extendable up to next two years based on performance.
Send out the detailed CV. All selected candidates will be informed.
No TA/DA will be provided. Interested candidates to send their cv to mohan.kumar@tdu.edu.in and hr@tdu.edu.in
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