NATIONAL INSTITUTE OF IMMUNOLOGY
NEW DELHI-110067
Applications are invited for the position of Research Associate for the following time-bound sponsored project as
per the details given below:
1. Project entitled “A novel vaccine
evaluation platform to support to SARSCoV-2 vaccine development in resourcelimiting setting" funded by BIRAC
Tenable till : 15.07.2022
Dr. Santiswarup Singha,
SS-IV
santiswarup@nii.ac.in
(The candidate may apply to
the above-mentioned email
address by and mention
“Research Associate” in the
subject of email.
Educational Qualifications: Ph.D in any branch of Life Sciences.
Emoluments: The selected candidates will draw consolidated emoluments as per DST OM dated 30.01.2019.
Rs. 47,000/- per month consolidated plus 24% HRA
Job description & Desired Knowledge:
The Research Associate will perform the experiments for producing peptide-major histocompatibility complex (pMHC)-tetrameter, labelled with flourochrome for characterizing antigen-specific T-cells.
The experiments will
have moderate-to-high complexity, including (but not limited to bacterial culture, transformation, cloning,
purification of plasmid DNA, cell culture for producing lentivirus, protein biochemistry, expression of p-MHC,
formulating p-MHC tetramer, animal handling, and flow cytometry. The Research Associate will also record,
analyze and plot the data to compile into the report for communication with PI in a planned and timely manner.
• Experience in bacterial culture, cloning, transformation, qRT-PCR and plasmid DNA isolation.
• Experience in cell culture, including (but not limited to) HEK-293T and Chinese hamster ovary (CHO) cells).
• Experience in working with lentivirus, transduction for making stable protein expression cell lime (preferably in
CHO cells).
• Sound knowledge in fast protein liquid chromatography (FPLC)-based protein purification techniques including
gel-filtration, affinity, and ion-exchange chromatography.
• Experience in characterizing the purified-protein using SDS/NATIVE PAGE, Western blot analysis.
• Experience with Flow cytometry
• Experience in optical, fluorescence microscopy
• Working knowledge of Immunology, mouse handling, including harvesting of tissues and collecting blood for
characterization of immune cells by flow cytometry.
• First author publications (minimum one) in Microbiology /Immunology /Biochemistry or related field.
Strong communication skill including advanced skills in Microsoft Office suite (Word, Excel, PowerPoint), data
analysis software such as GraphPad prism/ Origin/SigmaPlot etc. and ability to work in a team.
LAST DATE OF RECEIPT OF APPLICATIONS: 29.11.2021
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