NEHU Hands-on Training on Techniques used in Rapid Amplification of cDNA Ends | December 1-7, 2015 ~ helpBIOTECH

03 November 2015

NEHU Hands-on Training on Techniques used in Rapid Amplification of cDNA Ends | December 1-7, 2015


Announcement A 7 day course with Hands-on Training On techniques used in Rapid amplification of cDNA ends

Dec. 1-7, 2015


Centre for Advanced Studies in Botany

Department of Botany

North Eastern Hill University

Shillong – 793022

Sponsored By:

Department of Biotechnology

Govt. of India New Delhi

Evidence regarding transcription of genome into several different RNAs has necessitated methods for independent high-throughput verification of transcriptional starting sites (TSSs). 5′-RACE PCR is a well-established method to amplify nucleic acid sequences from a mRNA template between a defined internal site and unknown sequences at either the 3' or the 5' -end of the mRNA, thereby facilitating mapping of the TSS and location of promoter elements. The protocol leads to the generation of a cDNA copy of the RNA sequence of interest, through reverse transcription followed by amplification of the cDNA copies by PCR. The first step in RACE is to use reverse transcription to produce a cDNA copy of a region of the mRNA. In this process, an unknown end portion of a transcript is copied using a known sequence from the center of the transcript. The copied region is bounded by the known sequence, and either the 5' or 3' end of the target sequence. The training programme being organized at Department of Botany, North Eastern Hill University, Shillong under the DBT programme on “Establishment of Establishment of State level Biotech Hub under special programme for NER” shall cover: RNA isolation and cDNA synthesis RACE-PCR including 5’- and 3-’ RACE DNA sequencing and sequence confirmation The workshop is designed for College teachers and young researchers who seek to extend their teaching and research skills in Molecular Biology.

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