Agri Biotech Foundation 
PJTS Agricultural University Campus 
Rajendranagar, 
Hyderabad 500030 
TS
Agri Biotech Foundation is organizing a six day hands-on training course
on 
Introduction to Molecular Entomology 
During 
7 to 12th August 2017 
Course intends to address the needs of post-graduates, doctoral students
and faculty in colleges and Universities who would like to employ molecular
biology and tools of biotechnology to address their research and teaching
assignments specially in the area of entomology. This course is aimed at
those who have had minimal exposure to basic biotechnology and
molecular techniques but have several challenging tasks in hand that could
be conveniently addressed using these tools. 
Primary focus of the course would be on hands-on exposure to techniques
such as DNA extraction from insects, development and use of different
molecular markers in taxonomy, diagnostics, population diversity, virulence
monitoring studies and other related areas. Participants will also be
exposed to use of biotechnological tools in areas of plant-insect interaction,
development of insect-resistant transgenic crops, transgenic insects for
pest management and development of disease free insects. This course
hopes to blend theory with practical knowledge of useful molecular
techniquesthat would prove useful to address several contemporary
problems in agriculture related to insect pests. 
The course is being organized in technical collaboration with 
Dr. Suresh Nair and Dr. Deepak Kumar Sinha 
Plant Insect Interaction Group
International Centre for Genetic Engineering and Biotechnology (ICGEB)] 
Aruna Asaf Ali Marg
New Delhi 110067 
Details of the course (Tentative) 
Day1 
Inauguration 
T1 Introduction to the course - JSB 
P1 DNA extraction from insect material – 1 VBP 
P2 DNA extraction from insect material – 2 VBP 
Day 2 
T2 Molecular markers and their utility - DD 
P3 Introduction to PCR -1 NS 
P4 Introduction to PCR – 2 MS 
Day 3 
T3 – Insect-plant interactions – JSB 
P5 – Plant resistance genes – detection :NS 
P6 - Insect virulence genes – detection : JVN 
Day 4 
T4 – Plant-insect-virus interactions –SRR
P7 – Virus detection in plants : VBP 
P8 - Virus detection in insects :VBP 
Day 5 
T5 - Mitogenome and metagenome of Insects – DKS 
P9 - Mitogenome based markers : DKS 
P10 - Microbiome – detection and significance : DKS 
Day 6 
T6 – Plant transformation for insect resistance - GM 
P11 - Insect resistance gene detection :MGK MS 
P12 - Bioinformatics How to use publically accessible sequence databases to mine,
visualize, analyze, integrate, and manage biological and genetic information to understand the
genome of other organisms specially economically important insects: JVN 
Valedictory function and certificate distribution
Additional talks by guest faculty may be added 
T – Talk; P - Practical
4 
Course fee
For each participant course fee will be Rs.5000/-.
Number of Seats and Eligibility
In all 16 seats will be available, to be filled on first come first serve basis.
However, preference will be given for M.Sc. or Ph.D. degree holders in
Entomology or Agricultural Entomology or any branch of life science with
specific project related to insects. Submit your application (proforma
given here under) along with a brief writeup (250 words) on your current
research interests and objectives. 
Participants are encouraged to bring
insects (different populations if possible) of their interest preserved in 100%
alcohol for working on their material and develop project proposals, if
desired. 

 
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