helpBIOTECH Happy to Announce:
National Training Programme On Recent Techniques In Proteome Analysis
March 03 – March 23, 2011
Sponsored By
National Agricultural Innovation Project
Organized By
Animal Biotechnology Centre National Dairy Research Institute
(Deemed University)
(Indian Council of Agricultural Research)
Karnal (Haryana)-132001 www.ndri.res.in
ABOUT THE COURSE
Animal Biotechnology Centre is organizing a National Training Programme on “Recent Techniques in Proteome Analysis” from March 03- March 23, 2011, which is being sponsored by National Agriculture Innovation Project (NAIP) under the NAIP sub-project “Analysis of Mammary Gland Transcriptome and Proteome during Lactation and Involution in Indigenous Cattle and Buffalo for Identification of Probable Mammary Biomarkers”. The aim of the training is to impart basic as well as advanced skills to work on “Gene and Protein Expression, Protein Purification and Proteomics” using various biotechnological tools.
COURSE CONTENTS
Introduction to protein science
Isolation of genes and proteins
Sequence and structure alignment methods
Primer designing
Molecular cloning and over expression of genes in heteologous systems
High resolution purification of proteins
Characterization of native and recombinant proteins
Protein staining methods: Coomassie blue, silver staining and CuCl2.
Western blotting
Concept & scope of Proteomics
High throughput proteomics
1-D and 2-D gel electrophoresis
Protein spots analysis
Protein identification by peptide fingerprinting & de novo sequencing:
Concepts
Differential-in-Gel analysis (DIGE)
Analysis of proteome data
Gene expression studies
Introduction to protein structure determination
Introduction to Transcriptome analysis and functional genomics
ELIGIBILITY This in-house training program is targeted at enhancing capabilities of Scientists / Faculties / Research Associates / Senior Research Fellows working in ICAR Institutes, CAUs, SAUs and in various NAIP Sub-Projects, who are working or plan to start work in the areas of gene and protein expression and proteomics. We will consider applications from faculties of non-ICAR institutes subject to availability of seats. Genuinely interested candidates may kindly apply for the training programme by sending the filled in
registration form forwarded through proper channel on or before 10th February 2011 positively. A brief bio-data may kindly be sent along with the registration form.
registration form forwarded through proper channel on or before 10th February 2011 positively. A brief bio-data may kindly be sent along with the registration form.
Address for correspondence:
Dr. Ashok Kumar Mohanty
Senior Scientist
Animal Biotechnology Centre
National Dairy research Institute
Karnal -132001Phone: 0184-2259538 (Office);
Fax- 0184-2250042, Mobile 09896915989
Email: ashokmohanty1@gmail.com
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