IIT Delhi/BD BioSciences Course on 3D Cell Culture & Tissue Engineering 2011

3D Cell Culture & Tissue Engineering

September 1st- 3rd, 2011

Introduction Conventional 2D cell culture system provides a convenient & inexpensive platform for cell based analysis. However, there are a growing number of  evidences which indicate that the 3D culture  system exhibits a microenvironment which is  closer to in vivo situation than the cells in 2D. Such  three dimensional systems are the basis of Tissue  Engineering research and are also attractive  models to address a number of fundamental  This workshop is intended to give expert  knowledge in three dimensional cell culture to be  applied in scaffolds & matrices, tissue  engineering, cancer biology, stem cell biology,  drug screening etc.  questions in any area utilizing in vitro cellular  models.

This workshop is intended to give expert knowledge in three dimensional cell culture to  be applied in scaffolds & matrices, tissue engineering, cancer biology, stem cell biology,
drug screening etc. 

Course Features The objective of the course is to familiarize the participants with the techniques of animal cell culture in 2D and 3D environment. They will also learn to use tissue engineering strategies and the use of various commercially available scaffolds  (from Collagen, PLA, Calcium Phosphate etc. to TM BD Matrigel) for this purpose.

This CEP-cum-workshop program will provide unique opportunity for the participants to spend their valuable time with the experts in the area from academics, clinical research and biotechnology industries.

Who can participate? This unique workshop is designed for the students, post docs, biomedical engineers, biomaterial-scientists, pharmaceutical scientists, clinicians, technical / project managers of biotechnology & pharmaceutical industries and others involved or interested in any but not limited to the following research areas:

Tissue Engg & Regenerative Medicine
Cancer and Tumor Biology
Stem Cell Biology
Screening in Drug Development

Cell and Tissue Culture Technologies
Immunology & Gene Therapy
Vascular Surgery, Reconstructive and Plastic Surgery
Drug Delivery and Targeting
Nanobiotechnology
Course Contents

2D v/s 3D animal cell culture

Scaffold Formation techniques & Characterization

1. Scaffold composition: natural & synthetic polymeric materials
2. Fabrication techniques : textile technology- based scaffolds, rapid prototyping, particle leaching, electrospinning etc.
3. Scaffold characterization

Cells and Cell Culture

1. Aseptic techniques for cell culture and
media preparation
2. 2D and 3D cell culture (cell source,
freezing and resuscitation of frozen cells,
maintaining a cell culture, harvesting cells, cell
counting etc.)

Bioreactors and Integration

Types of reactors- spinner flask, rotating
vessel, perfusion bioreactor

Assays

1. SEM, MTT analysis
2. RT-PCR, real-time DNA amplification assay
3. FACS
4. Histology- Hematoxylin & Eosin, Alcian blue, Alizarin Red, Oil Red-O Staining

No. of seats: 15

How to apply?

Copy the enclosed form or type in the same format or download from IITD's website (http://www.iitd.ac.in/events/conf.html) and send the completely filled form either preferably through e-mail (scanned) or post it to the course coordinator. The subject of e-mail should be “2nd CEP-workshop on 3D cell culture & Tissue Engineering”. The same should be written on the envelope in case of sending hard copy.

Last date of receiving the completely st filled forms is 8th August 2011

The selected candidates will be contacted by email and will be asked to deposit the fees failing which wait listed candidates will be given the chance.

Fees:

Industry Participants: Rs 15,000/-
Academia Participants: Rs. 10,000/-

The fee is to be paid in advance through a Demand Draft or Cheque in favour of “Registrar, IIT Delhi” and should be sent to the course coordinator at the earliest but not later than 20 August, 2011. The fee includes course materials, accommodation, lunches and refreshments during the workshop.