CENTRAL DOGMA Pune Offering Project Training for BSc/MSc Students

CENTRAL DOGMA PRIVATE LIMITED

CIN: U74900PN2014PTC151301

Office: A4,Gulmohar, Behind Karnataka Bank, Baner, Pune 411045

Tel: +91- 9890038204  Email: anjali@centraldogma.co.in, info@ centraldogma.co.in

Carry out your Dissertation projects (Master’s and Bachelor’s) with us.

· Various project topics in Molbio/Microbio/ FoodTech/Plant Biotech/Environmental Microbiology

· Hands-on Training on project related techniques free when you enrol.

Enrolment started!!!!!!!

Call: 9890038204 / 9421973613

Upcoming one week workshop

Fundamental Techniques in Molecular Biology        

           

Techniques Covered

• Basic concept of molarity, normality and percentage, Buffer preparation
• Plasmid DNA isolation from bacterial cells
• Genomic DNA isolation from  buccal cells
• PCR along with theory of primer design
• Restriction digestion of plasmid
• RFLP
• Competent cell preparation, Transformation by CaCl method.

Fundamental Techniques in Microbiology   

   

Techniques covered:

• Basics of microbiology: Sterilization techniques, Media preparations, Variety of stainings- Monochrome staining, Gram staining, fungal staining
• Determination of TVC/CFU of a sample using spread plate and pour plate technique
• Isolation of bacteria, study of its colony characters and its identification upto genus level (will include performing various biochemical tests)
• Study of clinically important media for bacteria like Proteus, Salmonella,  Pseudomonas aeruginosa, Staphylococcus aureus
• Determination of MIC and MBC of an antibiotic                  

Fundamental Techniques in Herbal Drug Technology

Techniques Covered:

• Preparation of plant extracts using wet and dry plant tissues
• Preparation of plant extracts using different methods of extraction and various solvents
• Qualitative analysis of phyto chemicals in all types of variants (Tannins, Saponins,etc.)
• Quantitative analysis of phyto chemicals in all types of variants
• Determination of antimicrobial activity of plant extracts
• Regulatory requirements for the new drug (Theory)

Fundamental Techniques in Food Technology

Techniques Covered:

• Qualitative and Quantitative estimation of Proteins in food sample
• Qualitative and Quantitative estimation of Carbohydrates in food sample
• Lactic acid determination in food samples
• Vitamin C Estimation in food samples
• Phenolics Estimation in food samples
• Detection of Microbial Contaminants in food samples

Fundamental Techniques in Fermentation Technology

                               

Techniques covered:

• Shake Flask level fermentation of recombinant protein (with induction)
• Assessment of axenic strain by gram staining and biochemical characterization of isolate
• Cell lysis, harvest of fermentation product, SDS-PAGE
• Study of growth kinetics of bacteria
• Theory on fermentation technique, fermentor design, factors affecting fermentation process, strain improvement, media optimization, Scale-up of fermentations

Fundamental Techniques in Environmental Microbiology  

                             

Techniques covered:

• Isolation of bacteria from natural samples and their biochemical characterization
• Determination of CFU/gm of soil samples and calculation of diversity indices
• Isolation of symbiotic/non-symbiotic  nitrogen fixing bacteria/phosphate solubilizing bacteria
•  Assessment of potability of water using by the MPN (most probable number) test for coliforms
• Settling velocity determination for air samples

FOR ALL  ABOVE  MODULES:

Duration: 1 week

Time: 3-4 hrs/day

Venue: Central Dogma Pvt Ltd's lab in Baner, Pune

Fees: Rs. 3800 + 18% GST

Salient features of the module

• Each experiment would precede with a lecture, focusing on understanding of the principles, importance of individual reagent and trouble shooting at various steps.

• The experiments would be conducted individually, thus helping  in building the student's confidence.

Registration:

• The registration has to be done atleast 2 days prior to the training. A registration fees of Rs. 500 will be applicable per student.

Specialized Modules

Bacterial and Fungal Identification Techniques      

                          

Techniques covered:

• Study of bacterial and fungal morphologies by staining
• Biochemical Characterization of bacteria as per Bergey's manual
• Slide culture of yeast an filamentous fungi
• 16SrDNA PCR for bacterial identification
• ITS PCR for fungal identification
• Analysis of sequenced PCR products using BLAS 

Molecular Techniques in Plant Biotechnology

Techniques covered:

• Genomic DNA extraction from plant tissues
• Plant gene amplification by PCR
• Agarose Gel Electrophoresis
• RFLP of plant genomic DNA
• Plant protein extraction and its analysis using SDS PAGE 

FOR  ABOVE TWO  MODULES:

• Duration: 1 week
• Time: 3-4 hrs/day
• Venue: Central Dogma Pvt Ltd's lab in Baner, Pune
• Fees: Rs. 4500 + 18% GST 

Salient features of the module:

• Each experiment would precede with a lecture, focusing on understanding of the principles, importance of individual reagent and trouble shooting at various steps.

• The experiments would be conducted individually, thus helping  in building the student's confidence.

Registration:

• The registration has to be done atleast 2 days prior to the training. A registration fees of Rs. 500 will be applicable per student.