GIAN-CEM3DIP Practical Course on Cryo Electron Microscopy and 3D Image Processing of Macromolecular Assemblies and Cellular Tomography | July 2016 ~ helpBIOTECH

08 April 2016

GIAN-CEM3DIP Practical Course on Cryo Electron Microscopy and 3D Image Processing of Macromolecular Assemblies and Cellular Tomography | July 2016

helpBIOTECH Hyderabad CSIR Class Room Coaching June 2017

The 1st Cryo Electron Microscopy and 3D Image Processing of Macromolecular Assemblies and Cellular Tomography (CEM3DIP)

Under MHRD Scheme on Global Initiative on Academic Network (GIAN)

The GIAN-CEM3DIP practical course will cover :
  • 3D Electron Microscopy: History and Basic Principles
  • Biological sample and specimen preparation methods for TEM
  • TEM- principles, image formation , particle detection, Fourier Transform, Convolution, CTF, PSF, Resolution and FSC
  • Image processing, Point group symmetries, Classification, Orientation and Heterogenity of particles
  • Getting the first model using Conical Tilt and Projection Matching
  • Single particle CryoEM and 3D reconstruction
  • Challenges of in-situ structural biology
  • Principles and applications of Cryo Tomography. Cellular Tomography and Subtomogram averaging.
  • Refinement of atomic models against CryoEM maps, Flexible fitting, Validation, Assessment and Data deposition.
  • Hybrid methods: Protein Crystallography, Computational methods and CryoEM

To apply for this workshop click here.

More Info: http://workshop.iisertvm.ac.in/cem3dip/

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