GIAN-CEM3DIP Practical Course on Cryo Electron Microscopy and 3D Image Processing of Macromolecular Assemblies and Cellular Tomography | July 2016 ~ helpBIOTECH

08 April 2016

GIAN-CEM3DIP Practical Course on Cryo Electron Microscopy and 3D Image Processing of Macromolecular Assemblies and Cellular Tomography | July 2016

The 1st Cryo Electron Microscopy and 3D Image Processing of Macromolecular Assemblies and Cellular Tomography (CEM3DIP)

Under MHRD Scheme on Global Initiative on Academic Network (GIAN)

The GIAN-CEM3DIP practical course will cover :
  • 3D Electron Microscopy: History and Basic Principles
  • Biological sample and specimen preparation methods for TEM
  • TEM- principles, image formation , particle detection, Fourier Transform, Convolution, CTF, PSF, Resolution and FSC
  • Image processing, Point group symmetries, Classification, Orientation and Heterogenity of particles
  • Getting the first model using Conical Tilt and Projection Matching
  • Single particle CryoEM and 3D reconstruction
  • Challenges of in-situ structural biology
  • Principles and applications of Cryo Tomography. Cellular Tomography and Subtomogram averaging.
  • Refinement of atomic models against CryoEM maps, Flexible fitting, Validation, Assessment and Data deposition.
  • Hybrid methods: Protein Crystallography, Computational methods and CryoEM

To apply for this workshop click here.

More Info: http://workshop.iisertvm.ac.in/cem3dip/

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