CENTRAL DOGMA Pune Offering Project Training for BSc/MSc Students ~ helpBIOTECH

29 November 2019

CENTRAL DOGMA Pune Offering Project Training for BSc/MSc Students



CENTRAL DOGMA PRIVATE LIMITED
CIN: U74900PN2014PTC151301
Office: A4,Gulmohar, Behind Karnataka Bank, Baner, Pune 411045
Tel: +91- 9890038204  Email: anjali@centraldogma.co.in, info@ centraldogma.co.in

Carry out your Dissertation projects (Master’s and Bachelor’s) with us.


· Various project topics in Molbio/Microbio/ FoodTech/Plant Biotech/Environmental Microbiology
· Hands-on Training on project related techniques free when you enrol.

Enrolment started!!!!!!!

Call: 9890038204 / 9421973613

Upcoming one week workshop

Fundamental Techniques in Molecular Biology        
           
Techniques Covered

  • Basic concept of molarity, normality and percentage, Buffer preparation
  • Plasmid DNA isolation from bacterial cells
  • Genomic DNA isolation from  buccal cells
  • PCR along with theory of primer design
  • Restriction digestion of plasmid
  • RFLP
  • Competent cell preparation, Transformation by CaCl method.
Fundamental Techniques in Microbiology   
   
Techniques covered:

  • Basics of microbiology: Sterilization techniques, Media preparations, Variety of stainings- Monochrome staining, Gram staining, fungal staining
  • Determination of TVC/CFU of a sample using spread plate and pour plate technique
  • Isolation of bacteria, study of its colony characters and its identification upto genus level (will include performing various biochemical tests)
  • Study of clinically important media for bacteria like Proteus, Salmonella,  Pseudomonas aeruginosa, Staphylococcus aureus
  • Determination of MIC and MBC of an antibiotic                  
Fundamental Techniques in Herbal Drug Technology

Techniques Covered:

  • Preparation of plant extracts using wet and dry plant tissues
  • Preparation of plant extracts using different methods of extraction and various solvents
  • Qualitative analysis of phyto chemicals in all types of variants (Tannins, Saponins,etc.)
  • Quantitative analysis of phyto chemicals in all types of variants
  • Determination of antimicrobial activity of plant extracts
  • Regulatory requirements for the new drug (Theory)
Fundamental Techniques in Food Technology

Techniques Covered:

  • Qualitative and Quantitative estimation of Proteins in food sample
  • Qualitative and Quantitative estimation of Carbohydrates in food sample
  • Lactic acid determination in food samples
  • Vitamin C Estimation in food samples
  • Phenolics Estimation in food samples
  • Detection of Microbial Contaminants in food samples
Fundamental Techniques in Fermentation Technology
                               
Techniques covered:

  • Shake Flask level fermentation of recombinant protein (with induction)
  • Assessment of axenic strain by gram staining and biochemical characterization of isolate
  • Cell lysis, harvest of fermentation product, SDS-PAGE
  • Study of growth kinetics of bacteria
  • Theory on fermentation technique, fermentor design, factors affecting fermentation process, strain improvement, media optimization, Scale-up of fermentations
Fundamental Techniques in Environmental Microbiology  
                             
Techniques covered:

  • Isolation of bacteria from natural samples and their biochemical characterization
  • Determination of CFU/gm of soil samples and calculation of diversity indices
  • Isolation of symbiotic/non-symbiotic  nitrogen fixing bacteria/phosphate solubilizing bacteria
  •  Assessment of potability of water using by the MPN (most probable number) test for coliforms
  • Settling velocity determination for air samples
FOR ALL  ABOVE  MODULES:

Duration: 1 week
Time: 3-4 hrs/day
Venue: Central Dogma Pvt Ltd's lab in Baner, Pune
Fees: Rs. 3800 + 18% GST

Salient features of the module

  • Each experiment would precede with a lecture, focusing on understanding of the principles, importance of individual reagent and trouble shooting at various steps.
  • The experiments would be conducted individually, thus helping  in building the student's confidence.
Registration:
  • The registration has to be done atleast 2 days prior to the training. A registration fees of Rs. 500 will be applicable per student.
Specialized Modules

Bacterial and Fungal Identification Techniques      
                          
Techniques covered:

  • Study of bacterial and fungal morphologies by staining
  • Biochemical Characterization of bacteria as per Bergey's manual
  • Slide culture of yeast an filamentous fungi
  • 16SrDNA PCR for bacterial identification
  • ITS PCR for fungal identification
  • Analysis of sequenced PCR products using BLAS 
Molecular Techniques in Plant Biotechnology

Techniques covered:

  • Genomic DNA extraction from plant tissues
  • Plant gene amplification by PCR
  • Agarose Gel Electrophoresis
  • RFLP of plant genomic DNA
  • Plant protein extraction and its analysis using SDS PAGE 
FOR  ABOVE TWO  MODULES:

  • Duration: 1 week
  • Time: 3-4 hrs/day
  • Venue: Central Dogma Pvt Ltd's lab in Baner, Pune
  • Fees: Rs. 4500 + 18% GST 
Salient features of the module:

  • Each experiment would precede with a lecture, focusing on understanding of the principles, importance of individual reagent and trouble shooting at various steps.
  • The experiments would be conducted individually, thus helping  in building the student's confidence.
Registration:
  • The registration has to be done atleast 2 days prior to the training. A registration fees of Rs. 500 will be applicable per student.

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