Workshop on Molecular Biology and Biotechnology Techniques
December 10-20, 2009
Central Institute of Fisheries Education
(Deemed University - ICAR)
Mumbai – 400 061
Course outline
- Genomic DNA isolation from blood and tissues
- Isolation of plasmid DNA
- Quantification of nucleic acids and separation of DNA on agarose gels
- DNA cloning : Restriction digestion, ligation, transformation and selection of recombinant clones
- RNA isolation and cDNA preparation
- Polymerase chain reaction and its applications
- PCR primer designing
- Cloning PCR products using T/A cloning
- RAPD and data analysis
- Microsatellite DNA markers and DNA separation on PAGE
- Using DNA sequence analysis software
- Separation of proteins on SDS - PAGE
- Detection of antigenic proteins by western blotting
Intake capacity: Fifteen participants
Workshop flyer
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