GBPUAT Summer Training 2010 on PLANT TISSUE CULTURE/MOLECULAR BIOLOGY/ IMMUNOLOGICAL TECHNIQUES

helpBIOTECH Happy to Announce:

Department of Molecular Biology & Genetic Engineering
G. B. Pant University of Agriculture & Technology (GBPUA&T)
Pantnagar – 263 145
Distt. Udham Singh Nagar, Uttrakhand

INVITES APPLICATION FOR SUMMER TRAINING (2010-11) ON

“HAND-ON TRAINING ON PLANT TISSUE CULTURE, MOLECULAR BIOLOGY AND IMMUNOLOGICAL TECHNIQUES FOR STRENGTHENING OF FOUNDATION IN BIOTECHNOLOGY”

UNIQUE FEATURES OF TRAINING

A strong emphasis on hands on laboratory session.
Faculty are more experienced in their respective area.
Limited enrollments with an individualized learning environment.
Curriculum and experienced speaker are carefully selected to emphasize on practical approaches.

Module I Plant Tissue Culture

How to maintain good laboratory Practices
Biochemical calculations
Laboratory Instruments, principle and their uses
Preparation of MS media
Callus induction from different ex plants
Regeneration of callus (Shoot and root development)
Micropropagation/ multiple shoot induction
Immobilization of cells

Module II Molecular Biology and Bioinformatics

Isolation of genomic DNA from different sources
Isolation of plasmid DNA
DNA Quantification
Agrose Gel Electrophoresis
purification of DNA fragments from agarose gel
Restriction digestion
Data Mining GENBANK
Blasting Sequence
Primer designing
Preparation of vector DNA
Preparation of Insert DNA
Purification of vector and insert from agarose gel
Ligation
Preparation of Competent cells

Module III Protein Purification Techniques

Buffer preparation
Extraction of protein from different sources
Protein Concentration Techniques
Dialysis
Precipitation Techniques
Column Chromatography
Quantification of protein
Separation of protein by SDS-PAGE
Use of Radioisotope techniques

Module IV Immunology

Immunization of Rabbit
Determination of antibody titre
Latex agglutination test

UNIQUE FEATURES OF TRAINING

A strong emphasis on hands on laboratory session.
Faculty are more experienced in their respective area.
Limited enrollments with an individualized learning environment.
Curriculum and experienced speaker are carefully selected to emphasize on practical approaches.
Cryopreservation somatic cells and meristamatic tissues
Transformation
Screening of clones using restriction digestion
Variants of PCR and Amplification of gene
RT-PCR
Rapid immunology diagnostic procedure for STDs
Radial immuno diffusion test
Quantitative precipitin assay
Indirect ELISA
Western Blotting

Duration

Four to six weeks (June 1st - July 15th, 2010)

ELIGIBILITY

Enrollment of the candidates in B.Sc. / B. Tech. (Biotech) / M. Sc. / M. Tech. (Biotech) programme

Bench Fee

Rs. 5,000.00/ for DBT sponsored Institute and Rs. 10,000.00 for non DBT sponsored institute.

HOW To APPLY

The candidate should send their Bio-data along with recommendation letter from Head of the Institute/Principal/Dean of the College in the office of Head MBGE, C.B.S.H , G. B. Pant University of Agriculture and Technology Pantnagar, District U.S. Nagar – 263145 (Uttrakhand) latest by April 21th 2010. The Incomplete applications or received after due date will not be considered.

FOR FURTHER CLARIFICATION YOU MAY CONTACT

Dr. Anil Kumar (Professor& Head)
Department ofMolecular Biology& Genetic Engineering
Coordinator, Pantnagar Biotech Programme
Ph. 05944-233898 (O), 9411195450 (M)
Fax. 05944-233473
e-mali: ak_gupta2k@rediffmail.com

Dr. B.R. Singh (Asstt Prof.)
Department ofMolecular Biology& Genetic Engineering
Ph.9412323616
e-mail: singhbr@rediffmail.com

Further Info on GBPUA&T Summer Training 2010 on PLANT TISSUE CULTURE, MOLECULAR BIOLOGY AND IMMUNOLOGICAL TECHNIQUES