Life Science Incubator, IKP Knowledge Park Training Programme
Life Science Incubator, IKP Knowledge Park aims at imparting training through Analytical Instrumentation Facility onsite. Using latest technologies in the area of Biotechnology, hands on training in molecular biology has been introduced. By providing hands-on experience on various techniques to the students, Life Science Incubator hopes to improve the knowledge and skill set of students thereby developing a pool of trained employees with transferable skills to the industry.
This two weeks programme provides advanced training using latest technologies with hands on experience and demonstrations. This program intends to bridge the gap between academic training and industry needs in the area of Biotechnology and Pharmaceutical Sciences, which are in need of skilled people with hands-on experience. Topics to be covered:
Scheme I- Hands on training in recombinant DNA technology and protein expression
Laboratory Experiments (hands on): Choose either (a) or (b) option :
(a) Hands on training in recombinant DNA technology
Lab Fundamentals and biochemical calculations
Preparation of media, reagents, plates
Sterilization solutions / materials
Amplification of gene by PCR
Agarose gel electrophoresis
Preparation of vector and insert DNA by restriction digestion
Purification of DNA fragment from agarose gel
UV Spectrophotometry (DNA quantification)
Ligation of vector and insert DNA
Preparation of Competent cells by Calcium chloride method
Transformation into bacteria (E. coli), screening of recombinant clones by PCR
SDS-PAGE Experimental design and result analysis.
(b) Hands on training in recombinant protein expression
Lab Fundamentals and biochemical Calculations
Preparation of media, reagents
Sterilization solutions / materials
Preparation of Competent cells by Calcium chloride method
Transformation into bacteria (E. Coli, BL21),
Over expression of recombinant protein (IPTG induction)
Checking protein expression by SDS-PAGE (MW determination and purity by SDS – PAGE)
UV Spectrophotometry (Protein quantification)
Western Blot
Purity checking by HPLC
Molecular weight determination by Mass spectrometry
Course Duration: 2 Weeks
Course Fee for Scheme I: Rs.10,000 + service tax Course
Scheme II- Advanced training on high end Analytical Instruments
Choose either (a) or (b) option :
a) NMR
b) DSC, TGA, Particle Size Analyser, Mass Spectrophotometer, FTIR Tentative
Duration: 2 Weeks
Course Fee for Scheme II: Rs.10,000 + service tax
Date: Starts from May 2015 Time: 09:30 A.M. to 06:00 P.M. Monday to Friday
Venue: Life Science Incubator- IKP Knowledge Park, Hyderabad.
Eligibility: B.Tech, B.Sc, B.Pharm, M.Sc, M.Pharm, M.Tech Course Structure: 30% Lecture and 70% Lab
Course fee: Rs.10, 000 (i.e. Rs.10, 000/- fee + Service Tax) per person for each scheme of the course.
Features & Benefits: - Certificate of Course Completion. - Mini Project work.
For Registration: Please contact,
Mr. Amit Tambvekar at training@ikpknowledgepark.com
Dr. Sangita Sen Majee at sangita@ikpknowledgepark.com
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